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Histologic Comparison of Regeneration in Human Intrabony Defects When Osteogenin Is Combined With Demineralized Freeze‐Dried Bone Allograft and With Purified Bovine Collagen
Author(s) -
Bowers G.,
Feiton F.,
Middleton C.,
Glynn D.,
Sharp S.,
Mellonig J.,
Corio R,
Emerson J.,
Park S.,
Suzuki J.,
Ma S.,
Romberg E.,
Reddi A.H.
Publication year - 1991
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1991.62.11.690
Subject(s) - regeneration (biology) , tendon , matrix (chemical analysis) , demineralized bone matrix , dentistry , biomedical engineering , chemistry , anatomy , medicine , microbiology and biotechnology , materials science , biology , chromatography , amplifier , dbm , optoelectronics , cmos
A bone‐inductive protein, osteogenin , has been isolated from long bones of humans and offers promise as a grafting material. Studies, however, suggest that osteogenin must be combined with a bone‐derived matrix in order to initiate bone differentiation. The purpose of this study was to determine if osteogenin combined with demineralized freeze dried bone allograft (DFDBA), a bone‐derived matrix, and with a bovine tendon‐derived matrix will enhance regeneration of intrabony defects in humans. The tendon‐derived maxtrix and DFDBA used alone served as controls. The ability of each material to form a new attachment apparatus was evaluated independently in submerged and nonsubmerged environments in 2 patient populations. Lymphocyte testing was performed to assess development of an immune reaction to osteogenin. The most apical level of calculus on the root served as the histologic reference point to measure regeneration. Biopsies were obtained at 6 months and regeneration was measured histomorphometrically by 2 blinded evaluators. Serial sections from 36 submerged defects in 8 patients and 50 nonsubmerged defects in 6 patients were submitted for statistical analysis. Mean results indicate that osteogenin combined with DFDBA significantly enhanced regeneration of a new attachment apparatus and component tissues in a submerged environment. DFDBA plus osteogenin and DFDBA alone formed significantly more new attachment apparatus and component tissues than either the tendon‐derived matrix plus osteogenin or the tendon‐derived matrix alone in both submerged and nonsubmerged environments. There were no significant differences between the tendon‐derived matrix plus osteogenin and the tendon‐derived matrix alone in either the submerged or nonsubmerged environment. Osteogenin does not impair normal lymphocyte blastogenesis at 6 months postsurgical challenge. J Periodontol 1991; 62:690–702 .