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In Vitro Chemotactic Response of Osteoblast‐Like Osteosarcoma Cells to a Partially Purified Protein Extract of Demineralized Bone Matrix
Author(s) -
Padley Richard A.,
Cobb Charles M.,
Killoy William J.,
Newhouse Nancy L.,
Boyan Barbara D.
Publication year - 1991
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1991.62.1.15
Subject(s) - chemokinesis , chemotaxis , osteoblast , chemotaxis assay , in vitro , matrix (chemical analysis) , trypsin , chemistry , cell culture , microbiology and biotechnology , cell , cell migration , biochemistry , biology , enzyme , chromatography , receptor , genetics
T he purpose of this investigation was to evaluate the chemotactic potential of a partially purified protein extract from bone matrix when tested against osteosarcoma cells with Osteoblast characteristics. The chemotactic response of ROS 17/2 cells to a lyophilized bovine bone extract purified to “Urist step eight” was evaluated in Boyden blind well chambers. A checkerboard design was employed to test cell migration against positive, negative, and no concentration gradients, thereby controlling the effects of chemokinesis and/or random migration on results. The results demonstrate that the partially purified protein extract from bovine bone matrix is chemotactic since more cells migrated to positive gradients than to negative gradients ( P <.01). The chemotactic effect was confirmed by an increase in cell migration toward positive gradients of the bone extract compared to cell migration in the presence of no gradient ( P <.01). When no gradient was present, the cells exhibited an increased response in the presence of equal concentrations of the bone extract ( P <.01) indicating a chemokinetic effect. The proteinaceous nature of the chemoattractant was confirmed by its susceptibility to trypsin digestion and heat exposure. J Periodontol 1991;62:15–20 .