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Stimulation of Lymphocytes in Vitro by Bacteroides intermedius and Bacteroides (Porphyromonas) gingivalis Sonicates
Author(s) -
RaberDurlacher J.E.,
Zeijlemaker W.P.,
Meinesz A.A.P.,
AbrahamInpijn L.
Publication year - 1990
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1990.61.4.217
Subject(s) - porphyromonas gingivalis , bacteroides , microbiology and biotechnology , stimulation , antigen , polyclonal antibodies , bacteroidaceae , in vitro , biology , immunology , t cell , immune system , bacteria , biochemistry , genetics , neuroscience
T he present study was designed to assess whether the in vitro stimulation of lymphocytes by sonicates of Bacteroides intermedius and Bacteroides (Porphyromonas) gingivalis is antigen specific or non‐specific. In addition, the role of T and B lymphocytes in these responses was assessed. Peripheral blood lymphocytes obtained from healthy volunteers were cultured in the presence of these bacterial preparations and the proliferative response was measured. In similar experiments the response of umbilical cord blood lymphocytes did not exceed background values. In limiting dilution experiments only 1:4000, 1:6800, and 1:8200 of the lymphocytes initially reacted to B. intermedius, which strongly argues for the antigen‐specificity of the response. Purified T cells, in the presence of monocytes, proliferated when stimulated with B. intermedius and B. gingivalis. As for B cell stimulation, the bacterial extracts were capable of inducing IgM production, which appeared to be T cell dependent. These findings support the notion that B. intermedius and B. gingivalis induce specific T cell activation; secondarily, a T cell dependent, polyclonal B cell activation may occur. J Periodontol 1990; 61:217– 223.