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Multi‐Center Clinical Evaluation of a Chairside Method for Detecting Certain Periodontopathic Bacteria in Periodontal Disease
Author(s) -
Loesche W.J.,
Bretz W.A.,
Lopatin D.,
Stoll J.,
Rau CF.,
Hillenburg K.L.,
Killoy W.J.,
Drisko C.L.,
Williams R.,
Weber H.P.,
Clark W.,
Magnusson I.,
Walker C.,
Hujoel P.P
Publication year - 1990
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1990.61.3.189
Subject(s) - treponema denticola , actinobacillus , bacteroides , microbiology and biotechnology , porphyromonas gingivalis , dental plaque , gingival and periodontal pocket , medicine , periodontal disease , dentistry , chemistry , bacteria , periodontitis , biology , genetics
T he association of bacteroides gingivalis , Bacteroides forsythus, Treponema denticola , and Actinobacillus actinomycetemcomitans among others with periodontal disease offers the opportunity for the development of diagnostic tests that are based upon the detection and/or quantification of one or more of these organisms or their by‐products in the plaque. Three of the putative periodontal pathogens namely, T. denticola, B. gingivalis , and B. forsythus , can hydrolyze the synthetic trypsin substrate, N‐benzoyl‐DL‐arginine‐2‐naphthylamide (BANA) forming a color reaction. The present investigation evaluated a commercially developed solid state assay for BANA hydrolysis that can be read after 15 minutes incubation at chairside. A total of 702 subgingival plaque samples were collected from 117 patients seen at four university dental clinics and placed on reagent cards. The color development on the cards was compared to the presence of T. denticola and B. gingivalis in the plaque, and with the clinical appearance of the sampled sites. This multi‐center study demonstrated that antibodies to B. gingivalis and T. denticola could detect these organisms by an ELISA in the majority of the subgingival plaque samples. Comparable information could be obtained when the same plaques were evaluated by the reagent card format for BANA hydrolysis. The ELISA and reagent card were comparable in their ability to distinguish between clinically healthy and diseased sites. Both diagnostic procedures detected the periodontopathogens in plaques from sites that were judged clinically healthy. The reagent card test has certain clinical advantages over the ELISA in that it is simple, can be performed quickly at chairside, and provides valuable information as to the pathogenicity of the microflora at discrete oral sites. Such information may be used in the detection of patients or intraoral sites at risk for periodontal disease. J Periodontol 1990;61:189‐196.