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Effect of Periodontal Dressings on Human Gingival Fibroblasts In vitro
Author(s) -
Eber Robert M.,
Shuler Charles F.,
Buchanan William,
Beck Frank M.,
Horton John E.
Publication year - 1989
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1989.60.8.429
Subject(s) - eugenol , serial dilution , in vitro , fibroblast , hemocytometer , in vivo , chemistry , cell counting , thymidine , dentistry , pharmacology , medicine , cell , pathology , biology , biochemistry , cell cycle , alternative medicine , microbiology and biotechnology , organic chemistry
I n vitro cytotoxicity studies of periodontal dressings have not generally produced a result consistent with in vivo observations. These prior in vitro studies have not used human intraoral cell lines. We tested the effects of two eugenol containing and two non–eugenol periodontal dressings on cultured human gingival fibroblasts (HGF) (ATCC #1292). Replicate HGF cultures grown in microtiter plates were exposed to stock, 1:4 and 1:16 dilutions of extracts made from each of the four periodontal dressings. The HGF cultures were pulse labelled with tritiated thymidine ( 3 HTdR) after 24, 48, and 72 hours. Incorporations of the labelled thymidine were measured using liquid scintillation counting and expressed as counts per minute. The results showed that undiluted extracts from all four periodontal dressings totally inhibited 3 HTdR uptake ( P < 0.05). The 1:4 dilution of eugenol dressings inhibited 3 HTdR uptake significantly more than non–eugenol dressings ( P < 0.05). Interestingly, at 72 hours the 1:16 dilution of the non–eugenol dressings caused significantly increased 3 HTdR uptake which was not observed with the eugenol dressings. The present results suggest that the use of a human fibroblastic cell line for testing the effects of periodontal dressings may provide information about the relative biological effects of these dressings. Using this cell line, we have found that eugenol dressings inhibit fibroblast proliferation to a greater extent than non–eugenol dressings.