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Tissue Interactions with Dentin Specimens after Demineralization Using Tetracycline
Author(s) -
Frantz Bryan,
Polson Alan
Publication year - 1988
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1988.59.11.714
Subject(s) - demineralization , tetracycline , dentin , dentistry , chemistry , medicine , antibiotics , enamel paint , biochemistry
G reater cell attachment to demineralized dentin has been associated with subsequent development of a fiber attachment system. The purpose of this study was to evaluate tissue interactions to dentin demineralized with different concentrations of tetracycline solution. Dentin specimens were obtained from beneath root surfaces covered by periodontal ligament. Each rectangular specimen had a face of root surface dentin and an opposite surface of pulpal dentin. Experimental specimens were treated with a tetracycline solution of either 200 mg/cc or 100 mg/cc for five minutes. The remaining group of specimens served as untreated controls. Specimens were implanted transcutaneously into incisional wounds on the dorsal surface of rats with one end protruding through the skin. Four specimens in each group were available for examination one and ten days after implantation. Histologie and histometric analysis of both root and pulpal surface of implants included counts of adhering cells, assessment of implant length within the connective tissue, and evaluation of connective tissue fiber relationships. In each group, specimens became severely extruded between days one and ten, the number of attached cells decreased, and a fiber attachment system did not develop. Tetracycline‐treated surfaces had greater numbers of attached cells at both time points compared to untreated controls. No differences were discernible relating to different tetracycline concentrations. It was concluded that tetracycline‐demineralized dentin provided a substrate that increased cell attachment; however, this enhanced response did not result in a connective tissue attachment.