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Viadent ® , Ethanol, and pH Effects upon Gingival Epithelial‐like Cells, in Vitro
Author(s) -
Barczynski John L.,
Fletcher Ronald D.,
Segal Alan H.,
Conway James C.
Publication year - 1987
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1987.58.9.622
Subject(s) - in vitro , ethanol , serial dilution , chemistry , antimicrobial , toxicity , cell , pharmacology , sanguinarine , dentistry , microbiology and biotechnology , biochemistry , biology , medicine , pathology , alternative medicine , alkaloid , organic chemistry , stereochemistry
Interest has recently been directed towards the use of antiplaque mouthrinses. Most published material concerns the antimicrobial effects of these agents rather than their effects upon oral tissue. This study was conducted to evaluate the effect of a sanguinarine‐containing mouthrinse called Viadent ® upon epithelial‐like gingival cells. The cells were grown for 24 hours in supplemented Earle's medium, with and without different Viadent ® dilutions. Cell counts were made with a hematocytometer. It was found that 50% of the cells were inhibited at 1.2% Viadent ® . In similar studies, it was found that 70% ethanol and two pH buffers were less toxic than Viadent ® . Exposure of preformed cell monolayers to Viadent ® also showed significant inhibition. The relative toxicity of different antiplaque agents may be compared using such cells as a model system. In conclusion, it was observed that Viadent ® significantly affected gingival cell growth in vitro , that viable cell numbers were greatly reduced by short time exposure, and that the toxic effect of Viadent ® could only partially be accounted for by ethanol content and/or pH.