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Ultrastructural and Immunocytochemical Characterization of Polymorphonuclear Leukocytes from Gingival Crevice in Man
Author(s) -
Saito Ichiro,
Komiyama Kazuo,
Moro Itaru,
Akachi Kazufumi,
Shiomi Noboru,
Lto Koichi,
Murai Seidai,
Umemura Shinichiro
Publication year - 1987
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1987.58.7.493
Subject(s) - vacuole , cytoplasm , phagocytosis , periodontitis , ultrastructure , immunocytochemistry , antibody , pathology , immunofluorescence , immune system , granulocyte , chemistry , staining , immunohistochemistry , biology , microbiology and biotechnology , immunology , medicine
Polymorphonuclear leukocytes from the gingival crevicular fluid (CF‐PMNs) of patients with generalized severe periodontitis were examined using electron microscopy and immunocytochemical techniques. CF‐PMNs were found to contain numerous phagocytic vacuoles. This suggests that CF‐PMNs actively phagocytized various substances from the environment. Immunocytochemical staining with FITC‐conjugated IgG, IgM, and IgA reagents and TRITC‐conjugated C3 reagent was applied to CF‐PMNs as well as peripheral blood PMNs incubated with cell‐free crevicular fluid. The cytoplasm of PMNs exhibited numerous granular foci of immunofluorescence. This finding suggests that these proteins were acquired from the environment by PMNs. The coincidental appearances of Immunoglobulins and C3 in a single cell were considered to be immune complexes phagocytized by CF‐PMNs in generalized severe periodontitis.