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Growth Inhibitory Effects of Endotoxins from Bacteroides gingivalis and intermedius on Human Gingival Fibroblasts in Vitro
Author(s) -
Layman Don L.,
Diedrich Dana L.
Publication year - 1987
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1987.58.6.387
Subject(s) - bacteroides , in vitro , lipopolysaccharide , bacteroidaceae , microbiology and biotechnology , growth inhibition , cell growth , cell culture , gel electrophoresis , biology , molecular mass , chemistry , biochemistry , bacteria , immunology , enzyme , genetics
Purified endotoxin or lipopolysaccharide from Bacteroides gingivalis and Bacteroides inlermedius caused a similar dose‐dependent inhibition of growth of cultured human gingival fibroblasts as determined by 3 H‐thymidine incorporation and direct cell count. Approximately 200 μ g/ml endotoxin caused a 50% reduction in 3 H‐thymidine uptake of logarithmically growing cells. Inhibition of growth was similar in cultures of fibroblasts derived from either healthy or diseased human gingiva. When examining the change in cell number with time of exposure in culture, the rate of proliferation was significantly suppressed during the logarithmic phase of growth. However, the cells recovered so that the rate of proliferation, although reduced, was sufficient to produce a cell density similar to the control cells with prolonged culture. The endotoxins were characterized by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The profiles of the Bacteroides endotoxins were different. B. gingivalis endotoxin showed a wide range of distinct bands indicating a heterogeneous distribution of molecular species. Endotoxin from B. intermedius exhibited a few discrete low molecular weight bands, but the majority of the lipopolysaccharides electrophoresed as a diffuse band of high molecular weight material. The apparent heterogeneity of the two Bacteroides endotoxins and the similarity in growth inhibitory capacity suggest that growth inhibitory effects of these substances cannot be attributed to any Polysaccharide species of endotoxin.