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Hydrogen Peroxide Inhibits Glucose Metabolism and Collagen Synthesis in Bone
Author(s) -
Ramp Warren K.,
Arnold Roland R.,
Russell James E.,
Yancey John M.
Publication year - 1987
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1987.58.5.340
Subject(s) - hydrogen peroxide , carbohydrate metabolism , metabolism , chemistry , biochemistry , endocrinology , medicine , dentistry
Effects of H 2 O 2 on bone were evaluated in an organ culture system. Tibiae from chick embryos were incubated for up to 3 days in culture medium containing 0.07 to 20 mM H 2 O 2 . Glucose metabolism was monitored by measuring lactate production and oxygen consumption, and collagen synthesis was determined by hydroxylation of proline. In addition to markedly inhibiting these parameters, H 2 O 2 also decreased bone weight and alkaline Phosphatase activity. Multiple exposures to H 2 O 2 were somewhat more effective than a single exposure. Since H2O2 inhibits bone at low concentrations in vitro , the results suggest that the potential for harmful effects of H 2 O 2 in the oral cavity should be investigated.