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Biosynthesis of Prostaglandins in Gingiva of Patients with Chronic Periodontitis
Author(s) -
Mendieta Carlos F.,
Reeve Charles M.,
Romero J. Carlos
Publication year - 1985
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1985.56.1.44
Subject(s) - arachidonic acid , periodontitis , chemistry , inflammation , prostaglandin , thromboxane , medicine , incubation , thromboxane b2 , biosynthesis , endocrinology , pathology , biochemistry , enzyme , platelet
This study was undertaken to determine the ability of inflamed and normal gingival tissues to synthesize Prostaglandins (PGs) from the precursor arachidonic acid. Thirteen samples of inflamed human gingival tissue and six samples of normal human gingival tissue were studied. The inflammation was characterized histologically. After incubation of the tissue with [ 14 C]arachidonate, PG metabolites were separated by thin‐layer chromatography and identified by comparison with co‐chromatographed standards. Inflamed gingival tissue synthesized significantly larger amounts, compared to normal tissue, of 6‐keto‐PGF 1α ( P < 0.05), thromboxane B 2 ( P < 0.01), PGD 2 ( P < 0.05), and PGA 2 ( P < 0.001). Some unidentified metabolites, possibly lipoxygenase products, were detected in significantly ( P < 0.001) larger amounts in inflamed than in normal tissue.