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Eubacterium brachy : Reactivity in In Vitro Bone Resorptive Bioassay
Author(s) -
Vincent Jack W.,
Falkler William A.,
Heath James R.
Publication year - 1984
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1984.55.2.93
Subject(s) - antigen , bioassay , in vitro , extracellular , microbiology and biotechnology , eubacterium , biology , chemistry , biochemistry , immunology , bacteria , genetics
R ecent studies have demonstrated an association of Eubacterium sp. with the subgingival microflora of patients with chronic periodontitis. One species, Eubacterium brachy , was evaluated to determine the possible mechanisms by which this microorganism may contribute to this disease. An extracellular antigen was identified in the culture supernatant which reacted with antibodies in human sera. This antigen was isolated by methanol precipitation and purified by gel filtration. The purified extracellular antigen was reacted in vitro with 45 CaCl 2 ‐labeled fetal rat bone in a bone resorptive bioassay. This antigen was shown to have a molecular weight of 170,000, to share a line of identity with a sonicated preparation of E. brachy whole cells and to result in increased 45 CaCl 2 release from fetal rat bones when cultures were exposed to the purified extracellular antigen at concentrations of 10 to 53 μ g/ml.