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Evaluation of the Immunogenicity of Freeze‐Dried Skin Allografts in Humans: Cell‐Mediated Response
Author(s) -
Gher Marlin E.,
Vernino Arthur R.,
Maluish Annette E.,
Strong Douglas M.
Publication year - 1982
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1982.53.5.325
Subject(s) - trypan blue , peripheral blood mononuclear cell , effector , immunology , immunogenicity , antigen , cell , medicine , in vitro , cell mediated immunity , chemistry , immune system , immunity , biochemistry
P atients receiving freeze‐dried skin (FDS) allografts were evaluated for cell‐mediated response by a lymphocytotoxicity test. Eleven patients received single or multiple FDS allografts from a donor typed for human leukocyte antigen (HLA). Heparinized blood samples were drawn before the procedure and at 2, 4, and 8 weeks after grafting. Mononuclear cells were isolated by Ficoll‐Hypaque gradients. Cell‐mediated lymphocytotoxicity tests were conducted, with mononuclear cells from the FDS allograft recipients used as effector cells. Phytohemagglutinin‐stimulated blast target cells of the same HLA type as that of the skin donor were labeled with chromium 51. Lympholysis was evaluated by measuring the amount of 51 Cr release after 18 hours' incubation with target/effector cell ratios of 1:100, 1:50, and 1: 25. Negative control wells contained target cells alone. Positive control wells contained target cells and effector cells sensitized in vitro against the target cells. Viability of effector cells was tested by trypan‐blue dye exclusion and response to phytohemagglutinin. None of the experimental blood samples showed 51 Cr release significantly greater than that shown by the negative controls. Our findings of no cell‐mediated response and the findings of a previous study showing no production of anti‐HLA antibody in response to FDS allografts indicate that allogeneic FDS is an immunologically safe material for use in periodontal surgical procedures.