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The Further Localization of Antibody on Cemental Tissue
Author(s) -
Everhart Donald L.,
Dahab Omnia,
Wolff Larry,
Stahl S. Sigmund
Publication year - 1982
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1982.53.3.168
Subject(s) - cementum , antibody , antigen , periodontal disease , immunofluorescence , dental cementum , gingivitis , gingival and periodontal pocket , tooth surface , periodontitis , medicine , chemistry , dentistry , pathology , immunology , dentin
I n order to increase our understanding of the immunologic aspects of periodontal disease, three groups of extracted human teeth were evaluated for the presence of antibody on the cementum surface. Antibody was demonstrated by direct immunofluorescence using monospecific antibody with human α, γ and μ chain activity. The cemental surfaces of teeth with no clinical evidence of periodontal disease or teeth with gingivitis demonstrated no antibody. On the other hand, teeth involved with severe periodontal disease demonstrated positive fluorescence on root surfaces within the pockets, indicating the presence of antibody. No fluorescence was seen below the deepest point of the pocket. Furthermore, we have been studying a component recovered from the surface of teeth exposed to a periodontal pocket. Inhibition studies between serum antibody, extracted antigen and the antigen and antibody on the tooth surface suggest that we are dealing with the same antigen‐antibody complex in all cases.