Premium
A Selective Medium for Actinobacillus actinomycetemcomitans and the Incidence of the Organism in Juvenile Periodontitis
Author(s) -
Mandell Robert L.,
Socransky Sigmund S.
Publication year - 1981
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1981.52.10.593
Subject(s) - actinobacillus , bacitracin , microbiology and biotechnology , malachite green , agar , agar plate , chocolate agar , dental plaque , biology , isolation (microbiology) , chemistry , periodontitis , dentistry , bacteria , antibiotics , medicine , genetics , organic chemistry , adsorption
A selective medium , malachite green bacitracin agar, was developed for the isolation of Actinobacillus actinomycetemcomitans from subgingival plaque of periodontally diseased patients. The medium consisted of Trypticase soy agar 40 gm/liter, bacitracin 128 μ g/ml, malachite green 8 μ g/ml and 5% defibrinated sheep blood. The medium, when incubated in an atmosphere of air plus 10% CO 2 for 5 days, permitted greater than 80% recovery of pure cultures of A. actinomycetemcomitans when compared with a nonselective medium. The most frequent contaminant in plaque samples from different clinical conditions was Haemophilus aphrophilus. Decomposition of H 2 O 2 was useful in differentiating these two species. Clinical studies employing the malachite green bacitracin medium revealed a significant association between the presence of the organism, A. actinomycetemcomitans and juvenile periodontitis.