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Evaluation of the Immunogenicity of Freeze‐Dried Skin Allografts in Humans
Author(s) -
Gher Marlin E.,
Williams John E.,
Vernino Arthur R.,
Strong Douglas M.,
Pelleu George B.
Publication year - 1980
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1980.51.10.571
Subject(s) - medicine , immunogenicity , surgery , antibody , dentistry , immunology
A study was undertaken to evaluate the antigenic potential of allogeneic freeze‐dried skin (FDS) when used as a grafting material and to examine the clinical results when FDS is used for the correction of mucogingival problems in humans. FDS was obtained from an HLAtissue‐type donor according to Navy Tissue Bank protocol. Allogeneic FDS grafts were performed to correct mucogingival problems. In addition, periosteal fenestration was used in conjunction with approximately one‐half of the grafts. Documentation with a standardized periodontal probe consisted of clinical measurements taken preoperatively and 8 weeks postoperatively. Blood samples were drawn prior to and at 1,2,3,5, and 8 weeks postsurgically. Testing of blood samples for anti‐HLA antibody was performed by use of a modified microcytotoxicity assay. Thirty‐one patients received 36 FDS grafts involving 148 tooth sites. The FDS allografts resulted in a significant ( P < 0.001) increase in the width of attached‐type tissue (mean gain = 3.80 mm). Clinically, there was no sign of graft rejection, and serologically, all serum samples tested were negative for the presence of anti‐HLA antibody. FDS grafts used for the treatment of mucogingival problems in humans are nonimmunogenic as evaluated for anti‐HLA antibody. The surgical procedure performed using FDS as a graft material resulted in a significant increase in the width of attached‐type tissue.