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Reversibility of estrogenic sex changes in zebrafish ( Danio Rerio )
Author(s) -
Larsen Mia G.,
Bilberg Katrine,
Baatrup Erik
Publication year - 2009
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1897/08-563.1
Subject(s) - zebrafish , danio , biology , courtship , sexual differentiation , human fertilization , courtship display , sex ratio , sexual maturity , physiology , phenotype , endocrinology , medicine , andrology , zoology , anatomy , genetics , population , gene , environmental health
Abstract Development of male zebrafish (Danio rerio) courtship behavior was studied following estrogenic disruption of sexual differentiation. Sixty zebrafish were exposed at 28C to 5 ng/L (nominal concentration) of 17α‐ethinylestradiol (EE 2 ) from the egg stage until adulthood at four months of age, resulting in a female‐biased sex ratio. Twenty‐five EE 2 ‐exposed phenotypic female zebrafish were subsequently held in clean water for eight months. During this period, eight phenotypic males developed. These phenotypic males demonstrated significant behavioral aberrations and a low fertilization rate compared to control males.