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Development of a new assay for evaluation of l‐3,4‐dihydroxyphenylalanine cytotoxicity in catalase‐mutant Escherichia coli
Author(s) -
Wang DaHong,
Horita Masako,
Tsutsui Ken,
Sano Kuniaki,
Sauriasari Rani,
Masuoka Noriyoshi,
Takemura Yoko,
Takigawa Tomoko,
Takaki Jiro,
Ogino Keiki
Publication year - 2008
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1897/07-617.1
Subject(s) - catalase , escherichia coli , mutant , oxidative stress , reactive oxygen species , biochemistry , dihydroxyphenylalanine , strain (injury) , cytotoxicity , chemistry , microbiology and biotechnology , biology , gene , in vitro , anatomy , neuroscience , dopamine
The present study aimed to assess whether a newly constructed, catalase‐deficient Escherichia coli strain that express mammalian catalase gene could be used to identify oxidative stress‐generating chemicals. We tested L‐3,4‐dihydroxyphenylalanine ( L ‐DOPA), a well‐known agent that induces reactive oxygen species. We found that L ‐DOPA exposure reduced the survival of catalase‐mutant E. coli in a dose‐dependent manner, especially in the strains with lower catalase activities, implying the usefulness of these strains in assessment of oxidative chemicals.