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Development and testing of a green fluorescent protein‐based bacterial biosensor for measuring bioavailable arsenic in contaminated groundwater samples
Author(s) -
Liao Vivian HsiuChuan,
Ou KunLin
Publication year - 2005
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1897/04-500r.1
Subject(s) - biosensor , bioavailability , arsenic , contamination , environmental chemistry , green fluorescent protein , chemistry , contaminated groundwater , escherichia coli , arsenic contamination of groundwater , microbiology and biotechnology , chromatography , biology , biochemistry , ecology , gene , environmental remediation , bioinformatics , organic chemistry
A green fluorescent protein (GFP)‐based bacterial biosensor for the detection of bioavailable As(III), As(V), and Sb(III) was developed and characterized. The biosensor strain Escherichia coli DH5α (pVLAS1) was developed based on the expression of gfp under the control of the ars promoter and the arsR gene of Staphylococcus aureus plasmid pI258. Strain DH5α (pVLAS1) responded mainly to As(III), As(V), and Sb(III), with the lowest detectable concentrations being 0.4, 1, and 0.75 μM, respectively, during a 2‐h exposure and 0.1 μM for all three metal ions with an 8‐h induction period. To assess its applicability for analyzing environmentally relevant samples, the biosensor was field‐tested on shallow‐well groundwater for which contaminant levels were known. Our results demonstrate that the nonpathogenic bacterial biosensor developed in the present study is useful and applicable in determining the bioavailability of arsenic with high sensitivity in contaminated groundwater samples, and they suggest a potential for its inexpensive application in field‐ready tests.