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Toward detoxifying mercury‐polluted aquatic sediments with rice genetically engineered for mercury resistance
Author(s) -
Heaton Andrew C. P.,
Rugh Clayton L.,
Kim Tehryung,
Wang Nianjie J.,
Meagher Richard B.
Publication year - 2003
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1897/02-442
Subject(s) - methylmercury , mercury (programming language) , oryza sativa , environmental chemistry , chemistry , contamination , mercure , biology , gene , bioaccumulation , ecology , biochemistry , analytical chemistry (journal) , computer science , programming language
Mercury contamination of soil and water is a serious problem at many sites in the United States and throughout the world. Plant species expressing the bacterial mercuric reductase gene, merA , convert ionic mercury, Hg(II), from growth substrates to the less toxic metallic mercury, Hg(0). This activity confers mercury resistance to plants and removes mercury from the plant and substrates through volatilization. Our goal is to develop plants that intercept and remove Hg(II) from polluted aquatic systems before it can undergo bacterially mediated methylation to the neurotoxic methylmercury. Therefore, the merA gene under the control of a monocot promoter was introduced into Oryza sativa L. (rice) by particle gun bombardment. This is the first monocot and first wetland‐adapted species to express the gene. The merA ‐expressing rice germinated and grew on semisolid growth medium spiked with sufficient Hg(II) to kill the nonengineered (wild‐type) controls. To confirm that the resistance mechanism was the conversion of Hg(II) to Hg(0), seedlings of merA ‐expressing O. sativa were grown in Hg(II)‐spiked liquid medium or water‐saturated soil media and were shown to volatilize significantly more Hg(0) than wild‐type counterparts. Further genetic manipulation could yield plants with increased efficiency to extract soil Hg(II) and volatilize it as Hg(0) or with the novel ability to directly convert methylmercury to Hg(0).