Open AccessPENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus
Author(s) -
Sri Puji Astuti Wahyuningsih,
Virid Gibson,
Alfiah Hayati
Publication year - 2016
Publication title -
el-hayah/jurnal el-hayah
Language(s) - English
Resource type - Journals
eISSN - 2460-7207
pISSN - 2086-0064
DOI - 10.18860/elha.v5i4.3476
Subject(s) - dose , testosterone (patch) , negative control , polysaccharide , biology , andrology , medicine , pharmacology , endocrinology , traditional medicine , biochemistry
The purpose of this research was to determine the effect of polysaccharide krestin ( PSK) on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of 15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of 60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.