Open AccessKopling Oksidatif Eugenol Menggunakan Ekstrak Enzim Peroksidase dari Akar Tanaman Sawi Hijau serta Uji Bioaktivitasnya
Author(s) -
Bayu Ardiansah,
Antonius Herry Cahyana,
Wahyudi Priyono Suwarso,
Rianti Maryana,
Siti Merly
Publication year - 2018
Publication title -
alchemy
Language(s) - English
Resource type - Journals
eISSN - 2460-6871
pISSN - 2086-1710
DOI - 10.18860/al.v5i3.3776
Subject(s) - eugenol , chemistry , brassica , methyl eugenol , nuclear chemistry , yield (engineering) , monomer , stereochemistry , traditional medicine , organic chemistry , botany , biology , materials science , polymer , pest analysis , tephritidae , medicine , metallurgy
Peroxidase has been successfully extracted and partially purified from Brassica juncea L. Czern root with protein content and specific activity of 0.747 mg/mL and 0.244 U/mg, respectively. Then, it was applied as biocatalyst for green oxidative coupling reaction of eugenol to produce an orange crystal, dimeric form of eugenol (84% yield). Structure elucidation was done by using FTIR, UV-Vis and GC-MS instruments. The optical activity of dimeric eugenol was found to have Ra-configuration. Furthermore, biological activity of the product as antioxidant has been evaluated resulting in IC50 values of 32.14 and 42.43 ppm for dimeric and monomeric eugenol, respectively.