Open AccessTHE RELATION BETWEEN THE CONTENT OF PERIPHERAL BLOOD NEUTROPHILS AND THE BLOOD RELEASED FROM THE SURFACE OF PSORIATIC PAPULA
Author(s) -
Сергей Леонидович Кашутин,
A. K. Sherstennikova,
V. S. Neklyudova,
V. I. Nikolaev,
Л. Л. Шагров,
S. V. Klyuchareva,
V. A Piryatinskaya
Publication year - 2018
Publication title -
rossijskij žurnal kožnyh i veneričeskih boleznej
Language(s) - English
Resource type - Journals
eISSN - 2412-9097
pISSN - 1560-9588
DOI - 10.18821/1560-9588-2018-21-1-35-39
Subject(s) - medicine , psoriasis , cd11a , immunology , venous blood , pathogenesis , peripheral blood mononuclear cell , immune system , pathology , integrin alpha m , cd18 , biology , in vitro , biochemistry
The functional capabilities of neutrophils go beyond the traditional understanding, as phagocytic cells. There are evidences that show the role of neutrophils in antigen presentation to monocytes and lymphocytes, as well as the ability to regulate functions of immune cells through cytokine production. The role of neutrophils in the pathogenesis of psoriasis is not entirely clear, in this connection, the aim of our study was to examine the content ratio of peripheral blood neutrophils and the blood released from the surface of psoriatic papules with the level of expression of adhesion molecules. Material and methods. Clinical and immunological examination of 82 patients with vulgar and exudative psoriasis in the progressing and stationary stage (39 women and 43 men) aged from 20 to 60 years was carried out. Duration of the disease was from 3 months to 10 years. The control group included 50 healthy individuals (28 women and 22 men) aged from 20 to 60 years. They had no chronic diseases or aggravation. The number of neutrophils was determined on the smear of peripheral blood, as well as blood obtained from the surface of psoriatic papule, defined as a phenomenon of spot bleeding of the psoriatic triad. The content of L-selectin (CD62L, FITC), LFA-1 (CD11a, FITC), LFA-3 (CD58, FITC), ICAM-1 (CD54, FITC), L-selectin was measured on a flow cytometer FC-500 from Beckman Coulter (USA) PECAM-1 (CD31, FITC) on neutrophils of venous blood. Results. In venous blood of patients with psoriasis, the number of 2- and 3-segment forms of neutrophils increased statistically significantly in comparison with the control group, but the concentration of neutrophils with 4 or more segments in the nucleus decreased. In the psoriatic papule, the concentration of neutrophilic leukocytes was significantly reduced in comparison with peripheral blood, while in the segmentogram the predominant 2-segment forms of neutrophils were found. In venous blood, in psoriasis patients a statistically significant decrease of neutrophils with a PECAM-1 molecule was revealed, with which the final phase of transmigration into the tissue is connected. Discussion. Thus, in patients with psoriasis a statistically significant increase in the number of 2-segmetrix neutrophil forms indicates their stability in the lesion