Open AccessACUTE INTESTINAL INFECTIONS IN AMBULATORY PRACTICE
Author(s) -
Marina A. Nikonorova,
I.P. Saldan,
Nina V. Karbysheva,
S. G Pak,
Olga Belaia,
E. A Bobrovsky,
Е. А. Yakimenko,
E. A Nemilostiva
Publication year - 2018
Publication title -
èpidemiologiâ i infekcionnye bolezni
Language(s) - English
Resource type - Journals
eISSN - 2411-3026
pISSN - 1560-9529
DOI - 10.18821/1560-9529-2018-23-2-85-88
Subject(s) - etiology , ambulatory , feces , medicine , polymerase chain reaction , salmonella , acute gastroenteritis , gastroenterology , outpatient clinic , virus , virology , microbiology and biotechnology , biology , bacteria , gene , biochemistry , genetics
There is presented the structure of acute intestinal infections (AIIs) at the outpatient stage of medical care in 135 outpatients (70 men and 65 women, aged from 15 to 55 years) visited the Health Center of Barnaul. In all patients, the mild course of gastroenteritis predominated (the stool frequency did not exceed 4-5 times a day, body temperature - 37.2 0C). The investigation of biological material from patients (feces) was carried out by polymerase chain reaction (PCR) with hybridization-fluorescent detection “AmpliSens® AII screen-FL”. The results showed the high efficiency of the test system used, as in 118 out of 135 samples (87%) there was found genetic material of different etiology, 66.1% were of the viral origin, among which rotaviruses and noroviruses prevailed, 25.4% of samples had bacterial origin (Salmonella prevailed) and 19.5% of mixed virus-viral or bacterial-viral etiology. The work showed both the high sensitivity and specificity of the PCR method in the etiological diagnosis of AII. Among the examined patients, AII of viral origin prevailed (66.1%).