Open AccessLEVELS OF GROWTH FACTORS AND THEIR RECEPTORS IN INTACT AND TUMOR TISSUES OF FEMALE MICE IN DYNAMICS OF THE MALIGNANT MELANOMA GROWTH
Author(s) -
Oleg I. Kit,
Elena Mikhaylovna Frantsiyants,
В. А. Бандовкина,
Irina V. Kaplieva,
Lidia K. Trepitaki,
Ludmila Ya. Rozenko,
Natalia D. Cheryarina,
Yulia A. Pogorelova,
А. В. Шульга
Publication year - 2017
Publication title -
rossijskij onkologičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2412-9119
pISSN - 1028-9984
DOI - 10.18821/1028-9984-2017-22-5-281-287
Subject(s) - melanoma , angiogenesis , growth factor , biology , receptor , fibroblast growth factor , cancer research , vascular endothelial growth factor , stroma , metastasis , transforming growth factor , medicine , epidermal growth factor , endocrinology , cancer , immunology , vegf receptors , immunohistochemistry
The formation and growth of the tumor are accompanied with the development of new vasculature providing the neoplasm with nutrients for its growth and metastasis. Main agents for these processes are VEGF family that can be activated by various ways, including insulin growth factor (IGF) effects, epidermal growth factors (EGF), transforming growth factors (TGF) and fibroblast growth factor (FGF). The study included female C57BL/6 mice (n = 40) with B16/F10 melanoma transplanted subcutaneously. Changes in levels of VEGF-A, VEGF-C, VEGF-D and their receptors R1, R2 in dependence on the activity of IGF-I, IGF-II, EGF and FGF21 were studied by ELISA in tumor, perifocal area and intact tissues in the dynamics of B16/F10 melanoma growth using standard test systems. VEGF-A and VEGF-C were first to be activated in intact tissue of female mice with transplanted tumors, even prior to the tumor formation, which created conditions for the growth and development of the malignant tumor stroma. Later development of B16 melanoma was accompanied by the enhanced expression of VEGF growth factors and receptors in tumor and surrounding tissues. Main factors triggering angiogenesis in all samples included IGF2 and IGF1 which levels in the dynamics of melanoma growth correlated with VEGF-A values in tumor, its perifocal zone and intact tissues, and with VEGF-C in non-malignant tissue. The study demonstrated the multifactor staged activation of neoangiogenesis not only in B16 melanoma tissue but in surrounding and intact tissues as well.