Open AccessDetection of Flowering Ability on Several Bulbs Shallot Sources by using Hd3a and Endogenous GA3 Analysis
Author(s) -
Eddy Triharyanto,
Djoko Purnomo,
Ahmad Yunus,
Samanhudi Samanhudi
Publication year - 2020
Publication title -
indian journal of agricultural research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.241
H-Index - 8
eISSN - 0976-058X
pISSN - 0367-8245
DOI - 10.18805/ijare.a-561
Subject(s) - sowing , bulb , biology , gibberellin , horticulture , randomized block design , forensic science , botany , genetics
Background: Problems shallot cultivation in tropical regions such as Indonesia, is low productivity. Planting material is one of the causes of low productivity. The planting material used is a bulb that has been used continuously. It is makes planting material susceptible to infectious diseases and viruses. Efforts can be made to reducing the attack of virus infections in the planting material of the bulbs is by using seeds. However, seed production in Indonesia is still low due to the difficulty of flowering and low viability of seeds. The aim of this research was to detect flowering ability from the source of bulbs flowering and non-flowering clumps using Hd3a primers and endogenous GA3, also the effect of age of bulbs to flowering of shallot. Methods: The study used a complete randomized block design. The treatment consisted of two factors with three replications. The first factor was source of bulbs flowering and non-flowering clumps. The second factor was age bulbs which consists of three levels, namely the 60, 80 and 90 days age bulbs. The contents of gibberellins was analyzed using HPLC analysis. Molecular analysis to determine the potential of flowering genes used SSR primer (Hd3a). Result: Hd3a primer can be used as a marker of flowering shallot. Source of bulbs flowering and non-flowering clumps have the same flowering potential gene. The percentage of flowering in flowering clumps was higher than non-flowering clumps and correlates with endogenous gibberellins content. The age of bulbs indicates that older bulbs gave a higher percentage of flowering.