Comparative investigation of DNA extraction methods in black gram (Vigna mungo (L.) | Zendy

M. Prakash | Zendy; Bhavana Priyadharshini | Zendy; M. Vignesh | Zendy; R. Anandan | Zendy

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Comparative investigation of DNA extraction methods in black gram (Vigna mungo (L.)

Author(s) -

M. Prakash,

Bhavana Priyadharshini,

M. Vignesh,

R. Anandan

Publication year - 2019

Publication title -

indian journal of agricultural research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.241

H-Index - 8

eISSN - 0976-058X

pISSN - 0367-8245

DOI - 10.18805/ijare.a-5212

Subject(s) - dna extraction , dna , agarose gel electrophoresis , gram , genotyping , genomic dna , vigna , chromatography , agarose , extraction (chemistry) , biology , microbiology and biotechnology , genotype , chemistry , polymerase chain reaction , bacteria , biochemistry , genetics , gene , botany

Isolation of intact, double stranded, pure and non- contaminated genomic DNA is prerequisite for large scale genotyping analysis including DNA-banks. Three methods of DNA isolation (Dellaporta, CTAB and Hi-PurAg DNA isolation kits) from 25 black gram genotypes were compared in terms of the yield, purity, integrity, and stability of extracted DNA. Purity and quantification of isolated DNA samples was confirmed by using the UV nano-spectrophotometer at OD260/280 and the same is confirmed based by agarose gel electrophoresis. The CTAB method showed the best results followed by Hi-PurAg and Dellaporta method. The CTAB DNA extraction method was found to be the most efficient DNA extraction method, capable of providing high quality, pure and stable DNA and could be used for various molecular related works. All the 25 black gram genotypes for this research gave good yield of DNA from the established modified CTAB protocol.

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