Bukhuldaria vietnamensis mediated induced wilt resistance in chickpea

The efficacy of root dipping with Bukhuldaria vietnamensis isolate KM2 (Bv-KM2) anantagonist of Fusarium oxysporum was evaluated to improve the tolerance mechanism of chickpea against wilt. The increase in activity of key ROS enzymes of root tissues viz., peroxidase, catalase and superoxide dismutase was reported with the increase in time course of infection in resistance and susceptible varieties at 1 and 5 DAI. The pre-treatment with Bv-KM2 helps to maintain the higher level of ROS enzymes in susceptible variety JG-62, hence imparts resistance against Foc stress. In resistant variety WR-315 it further improves resistance towards Foc stress through enhanced production of ROS enzymes. The role of phenolic compounds and polyamines was studied from root tissues using HPTLC at 5 DAI. Among the polyphenols, ferulic acid was found to be constitutively expressing in both varieties over the treatment condition, the salicylic acid was found in resistant variety WR-315 over the treatment whereas it was found to be induced in susceptible variety JG-62. Four polyamines were detected through HPTLC of which two were unidentified where as two were identified as cadvarine and spermine. One of unidentified polyamiens (P2) was reported only in resistant variety WR-315 under different treatment conditions. Overall the Bv-KM2 treatment leads to alteration in ROS enzymes, polyphenols and polyamines which result in improved tolerance to Foc in both resistant and susceptible varieties.