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Prevalence study of Salmonella spp. in milk and milk products
Author(s) -
Pooja Singh,
R. V. Singh,
Bhavana Gupta,
Sabya Sanchi Tripathi,
Kewal Singh Tomar,
Sachin Jain,
Y.P. Sahni
Publication year - 2018
Publication title -
asian journal of dairy and food research
Language(s) - English
Resource type - Journals
eISSN - 0976-0563
pISSN - 0971-4456
DOI - 10.18805/ajdfr.dr-1252
Subject(s) - salmonella , nalidixic acid , raw milk , norfloxacin , food science , cefotaxime , ciprofloxacin , biology , ofloxacin , microbiology and biotechnology , chloramphenicol , tetracycline , veterinary medicine , chemistry , antibiotics , medicine , bacteria , genetics
The present study was carried out to observe the presence of Salmonella in milk and milk products. The investigation of 210 samples revealed presence of 7.61% Salmonella spp. with higher prevalence in raw milk and dahi - 11.9% each , followed by ice-cream -9.52% and khoa - 4.76%. None of the sample of flavored milk showed Salmonella. In molecular study, 5 (31.2%) isolates were found positive for invA gene. The gene was present in raw milk - 18.75% and 6.25% in khoa and ice-cream each. Salmonella isolates were found sensitive to netillin - 100%, ofloxacin - 87.5%, norfloxacin, ciprofloxacin and nalidixic acid – (62.5% each), streptomycin and amikacin – (56.2% each). The isolates were resistant to nitrofurantoin- 81.2%, tetracycline -75.0%, co-trimoxazole - 68.7%, ampicilline - 56.2% and cefotaxime - 50.0%. The observation displayed unhygienic atmosphere prevailing during production of dairy products which lead to contamination of Salmonella and thus may cause public health problem to the consumers.

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