Open AccessPCR based detection and Biofilm formation of Salmonella from fresh coriander leaves (Coriandrum sativum)
Author(s) -
S. Leonal Rabins,
Abhishek Bhattacharya,
V.J. Ajay Kumar,
C. Vijayan
Publication year - 2018
Publication title -
journal of dairying, foods and home sciences/journal of dairying foods and home sciences
Language(s) - English
Resource type - Journals
eISSN - 0976-0563
pISSN - 0971-4456
DOI - 10.18805/ajdfr.dr-1158
Subject(s) - salmonella , microbiology and biotechnology , biology , coriandrum , antimicrobial , norfloxacin , ofloxacin , biofilm , cefotaxime , ciprofloxacin , multiple drug resistance , antibiotic resistance , drug resistance , antibiotics , sativum , bacteria , horticulture , genetics
The aim of the study was to detect Salmonella from coriander leaves by polymerase chain reaction, determine biofilm formation and antimicrobial resistance profiling of the isolates. Four Salmonella isolates were recovered from the total of 60 samples. The isolates were confirmed by PCR. The confirmed isolates were subjected for biofilm production assay and showed that all Salmonella isolates were able to form biofilm. Biofilm forming isolates were also subjected for antimicrobial resistance profiling. Three isolates were showed sensitivity against norfloxacin (92.5%). One was showing sensitivity against cefotaxime (90%). All the four isolates were showing complete resistant to ciprofloxacin, nalidixicacid, chloramphenical, gentamicin and cephalexin. Clindamycin and ofloxacin were showed sensitivity 20%. The finding of this study shows that coriander leaves are potential host for the transmission of Salmonella to human and animals. The ability of the isolates to form biofilm reveals the potential of the isolates to persist on the green leafy vegetables and the pathogenic status of the isolates as well as ability to resist antimicrobial chemotherapy