Open AccessRelationship between In vivo, In vitro Parameters and Chemical Composition to Predict the Nutritive Value of Some Legume Forages
Author(s) -
Alexandre Ferro Aissa,
C. Ben Mustapha,
M. R. Alvir,
M. Hadj Ayed,
Ibrahim El Akram Znaïdi,
Muhammad Nizar,
K. Kraïem
Publication year - 2021
Publication title -
agricutural science digest/agricultural science digest
Language(s) - English
Resource type - Journals
eISSN - 0976-0547
pISSN - 0253-150X
DOI - 10.18805/ag.d-252
Subject(s) - forage , dry matter , in vivo , zoology , legume , medicago sativa , trifolium alexandrinum , biology , ruminant , incubation , chemical composition , chemistry , food science , biochemistry , botany , agronomy , microbiology and biotechnology , pasture , organic chemistry
Background: Many in vivo and laboratory methods have been used to evaluate ruminant feeds. The objective of this experiment was to determine feed intake, digestibility, kinetics of gas production, in vitro organic matter digestibility (OMDv) and metabolizable energy (ME) of Trifolium alexandrinum (T. alexandrinum) and Medicago sativa (M. sativa) at different maturity stages through in vivo and in vitro methods to elaborate predictive equations from chemical constituents. Methods: In vivo assay was carried out with two groups of five rams male kipped in metabolism cage. Samples of forage, refusal and feces were collected and processed for chemical analysis. In vitro gaz production technique was performed on forage samples.Result: The bud and early bloom stage of M. sativa recorded the highest digestibility values of the chemical component (P less than 0.05). The OMDv of M. sativa was 60.65 and 68.26% (P less than 0.01) for early bloom stage and bud stage, respectively. Crude protein digestibility (CPD) was positively correlated with crude protein rate (R2=0.83, P less than 0.05). The gas production from the insoluble fraction “b” fraction showed a positive correlation with acid detergent fiber (ADF) (R2=0.999, P less than 0.001). However, cumulative gas production at 24h and 48 h of incubation were negatively correlated (P less than 0.05) with the ADF rate (R2= -0.98 and -0.97, respectively).