Analysis of impact of anaerobic condition on the aflatoxin production in Aspergillus parasiticus Speare

Aflatoxins are the natural carcinogens that are the best characterized as fungal secondary metabolites. The producers that are responsible for aflatoxin biosynthesis are strongly associated in toxic contamination of essential agriculturalproducts. Aspergillus parasiticus is an exclusive fungus that participates in causing hepatic problems in humans and cattle. These mycotoxins are greatly influenced by abiotic stresses. The fungal growth, proliferation and its toxigenicity are highlyinfluenced by these stresses. Present study aimed to restrict the mycelial growth and to prevent aflatoxin preparation in A. parasiticus under the anoxic stress. The monosporic strains of A. parasiticus were grown in two different Erlenmeyer conicalflasks containing Czapek Dox Broth and Czapek Dox Agar under both aerobic and anaerobic conditions. The anoxic condition was maintained using Anaero Bag System. Aflatoxin was isolated after 10 days, and quantitative estimation was done by using HighPerformance Liquid Chromatography (HPLC). The experimental outcome showed that there was a drastic decrease in both the morphological growth and the aflatoxin biosynthesis of A. parasiticus in anoxic state.