Open AccessFirst report of Alternaria alternata causing the golden spot in xoconostle (Opuntia matudae) in Hidalgo, México
Author(s) -
David Montiel-Salero,
Angélica Jímenez-Aguilar,
Susana E. Ramírez-Sánchez,
Daniel Ruiz-Juárez,
Octavio Guerrero-Andrade,
Eva Segundo-Pedraza,
Javier L. Olivares-Orozco,
Miguel Ángel Ramos-López,
Antonio Flores-Macías
Publication year - 2021
Publication title -
revista mexicana de fitopatología(en línea)/revista mexicana de fitopatología
Language(s) - English
Resource type - Journals
eISSN - 2007-8080
pISSN - 0185-3309
DOI - 10.18781/r.mex.fit.2107-3
Subject(s) - alternaria alternata , alternaria , leaf spot , horticulture , crop , biology , phyllosphere , inoculation , botany , veterinary medicine , agronomy , medicine , bacteria , genetics
The species Opuntia ficus-indica is affected by pests and diseases, one of the most important of these being golden spot. The latter has been reported in various countries, including Mexico. Symptoms similar to those of golden spot in O. ficus-indica have been observed in the xoconostle crop ( Opuntia matudae Scheinvar, cv. Rosa). The objective of this study was to isolate and identify the causative agent that causes the symptoms, which resembled those of the disease described as golden spot in O. ficus-indica . Simple random sampling in the field (10 plots) was carried out on xoconostle plants that presented the described symptoms. Slices of diseased plant tissue were cut and placed in Petri plates with PDA medium. Compliance with Koch’s postulates showed that the isolated (one aislated) of Alternaria sp. obtained from the field samples colonized the cladodes after inoculations in the greenhouse. Through taxonomic keys, Alternaria sp. was identified as the causative microorganism. Molecular characterization of the isolated Alternaria sp. was identified as Alternaria alternata . This is the first report worldwide of Alternaria alternata as the causal agent of golden spot in a xoconostle crop.