Comparison of various decalcificators in preparation of DNA from human rib bones | Zendy

Magdalena Żołędziewska | Zendy; Stanisław Gronkiewicz | Zendy; Tadeusz Dobosz | Zendy

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Comparison of various decalcificators in preparation of DNA from human rib bones

Author(s) -

Magdalena Żołędziewska,

Stanisław Gronkiewicz,

Tadeusz Dobosz

Publication year - 2002

Publication title -

anthropological review

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.262

H-Index - 12

eISSN - 2083-4594

pISSN - 1898-6773

DOI - 10.18778/1898-6773.65.07

Subject(s) - bone decalcification , dna , calcium , chemistry , sodium , sodium citrate , biochemistry , biophysics , mineralogy , nuclear chemistry , biology , organic chemistry , dentistry , pathology , medicine

Various calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantities of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and of Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and – in two-step decalcification – sodium citrate.

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