Open AccessComparison of various decalcificators in preparation of DNA from human rib bones
Author(s) -
Magdalena Żołędziewska,
Stanisław Gronkiewicz,
Tadeusz Dobosz
Publication year - 2002
Publication title -
anthropological review
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.262
H-Index - 12
eISSN - 2083-4594
pISSN - 1898-6773
DOI - 10.18778/1898-6773.65.07
Subject(s) - bone decalcification , dna , calcium , chemistry , sodium , sodium citrate , biochemistry , biophysics , mineralogy , nuclear chemistry , biology , organic chemistry , dentistry , pathology , medicine
Various calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantities of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and of Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and – in two-step decalcification – sodium citrate.