Open AccessCharacterization of mesenchymal heart cells obtained from patients with tetralogy of Fallot and ventricular septal defect
Author(s) -
И. А. Козырев,
А. С. Головкин,
Е. С. Игнатьева,
Pavel Dokshin,
Е. В. Грехов,
М. Л. Гордеев,
Tatiana Pervunina,
Anna Kostareva,
Anna Malashicheva
Publication year - 2019
Publication title -
translâcionnaâ medicina
Language(s) - English
Resource type - Journals
eISSN - 2410-5155
pISSN - 2311-4495
DOI - 10.18705/2311-4495-2019-6-5-16-23
Subject(s) - tetralogy of fallot , mesenchymal stem cell , cd90 , medicine , cardiology , pdgfrb , cd34 , cd117 , pathology , stem cell , heart disease , microbiology and biotechnology , biology , biochemistry , gene
Objective. Phenotypic analysis of cardiac mesenchymal cells from patients with a ventricular septal defect and tetralogy of Fallot. Methods. The study included cardiac mesenchymal cells from 8 patients with ventricular septal defect and from 18 patients with tetralogy of Fallot who underwent surgery. Using the flow cytometry method, the content of the following antigens on the cell surface was evaluated: CD31, CD34, CD90, CD117, CD146, CD166, PDGFRB. Results. Cardiac mesenchymal cells from patients with ventricular septal defect are statistically significantly different from patients with tetralogy of Fallot in surface markers CD90 and PDGFRB. The content of a stem cell marker PDGFRB tends to decrease with age in patients with tetralogy of Fallot. Conclusion. The cells obtained from myocardial tissue from patients with tetralogy of Fallot and from patients with ventricular septal defects have characteristics of mesenchymal stem cells. The differences found between the cells from the two groups of patients indicate that pathology can affect the phenotype of cardiac mesenchymal cells. Apparently, the stem properties of the mesenchymal heart cells tend to decrease with age.