Open AccessNAP-22 and MARCKS mRNA expression levels in spontaneously hypertensive rat kidneys: the effect of drinking water with different calcium contents
Author(s) -
A. S. Aldekeeva,
A. Yu. Plekhanov,
N. Z. Klyueva
Publication year - 2021
Publication title -
arterialʹnaâ gipertenziâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.126
H-Index - 5
eISSN - 2411-8524
pISSN - 1607-419X
DOI - 10.18705/1607-419x-2021-27-4-
Subject(s) - nap , calcium , endocrinology , medicine , messenger rna , kidney , calcium in biology , spontaneously hypertensive rat , chemistry , intracellular , biology , blood pressure , biochemistry , gene , neuroscience
Objective. The aim of study was to assess the effect of the level of calcium intake with drinking water on NAP-22 and MARCKS mRNA expression in cortical and medullar kidney layers of spontaneously hypertensive rats. Design and methods . The study involved 90-day-old SHR (n = 8) and WKY (n = 8) strain rats of both sexes. We assessed tissue samples from cortical and medullar kidney layers. NAP-22 and MARCKS mRNA expression levels were determined by RT-PCR. Results. Sufficient drinking water calcium intake was associated with similar the expression of NAP-22 and MARCKS mRNA in kidneys of spontaneously hypertensive and normotensive rats. Consumption of drinking water with insufficient calcium content it decreases in both rat strains, being more evident in spontaneously hypertensive rats, especially in the medullar layer. Conclusions. Our results show that genetically determined impairments of calcium metabolism in cells of spontaneously-hypertensive rats (SHR line) and their effect on intracellular signaling processes are more evident with the reduced intake of exogenous calcium.