Open AccessEffect of the NO donator nitrosothiol glutathione on the rat blood levels of nitric oxides and malonic dialdehyde
Author(s) -
L. Yu. Kaminskaya,
А. А. Жлоба,
Л. А. Александрова,
O. M. Moiseyeva,
V. L. Emanuel,
Ye. V. Shlyakhto
Publication year - 2005
Publication title -
arterialʹnaâ gipertenziâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.126
H-Index - 5
eISSN - 2411-8524
pISSN - 1607-419X
DOI - 10.18705/1607-419x-2005-11-1-5-9
Subject(s) - ferrous , chemistry , oxidative stress , lipid peroxidation , nitric oxide , glutathione , malonic acid , biochemistry , pharmacology , chromatography , organic chemistry , medicine , enzyme
Nitric oxide (NO) is a paramagnetic molecule, îe a free radical, and, under poor metabolic conditions, can induce the so-called nitrosylating stress HPLC was used to study an increase of the lipid peroxidation (LPO) product malonic dialdehyde when the NO donor mtrosoglutathione was administered to experimental rats Chromatographic analysis was made on an Agilent-1100 liquid chromatograph (Agilent Technologies, Germany) The effect of mtrosogluthatione was compared with that of a mixture of ferrous mtrile acetate and homocystine given to the animals as a prooxidant Under experimental oxidative stress after administration of ferrous mtrile and homocystine (40 mkmole/kg, calculated with reference to iron), the rat blood serum level of malonic dialdehyde increased by approximately 20 times as compared to that in the control group of animals Four-fold activation of malonic dialdehyde formation was revealed in rats under nitrosothiol oxidation (after administration of mtrosogluthatione in a dose of 200 mkmole/kg) It is concluded that NO donors are able to induce oxidative stress When NO donors are used in long-term therapy, it is necessary to control the body’s oxidative status