Open AccessVariability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed
Author(s) -
Л. А. Храброва,
N. V. Blohina,
Б. З. Базарон,
Т. Н. Хамируев
Publication year - 2021
Publication title -
vavilovskij žurnal genetiki i selekcii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.188
H-Index - 7
eISSN - 2500-0462
pISSN - 2500-3259
DOI - 10.18699/vj21.055
Subject(s) - haplogroup , mitochondrial dna , d loop , breed , haplotype , biology , hypervariable region , human mitochondrial dna haplogroup , subclade , genetics , genetic diversity , horse , evolutionary biology , phylogenetic tree , clade , genotype , gene , population , demography , sociology , paleontology
The Zabaikalskaya horse is an indigenous breed of horses from Siberia with diverse use. It is characterized by endurance and good adaptability to year-round herd maintenance in the harsh conditions of the Baikal steppes. To determine the genetic characteristics of the maternal lineage of the Zabaikalskaya horse breed based on mitochondrial DNA polymorphisms, we collected hair samples from 31 horses belonging to breeding farms in the Trans-Baikal Territory. Analysis of the 530 bp sequence of the mtDNA D-loop was performed using the maximum composite likelihood (MCL) model in combination with bootstrap analysis. When studying the polymorphism of the hypervariable region of the mtDNA D- loop in Zabaikalskaya horses, we identif ied 31 haplotypes representing 8 haplogroups: B, C, G, H, L, M, Q and R according to modern classif ication. The sequenced fragment of the D-loop from nucleotide position 15471 to 16000 contained 17 polymorphic sites, mainly represented by the A→G, G→A and T→C transitions. The haplogroups Q (25.81 %), B (19.35 %), G (16.13 %) and H (12.90 %) were prevailing in the mtDNA structure of this breed. Genetic analysis of the mitochondrial genome of the Zabaikalskaya horse revealed a high level of diversity of haplotypes and haplogroups, which are typical for the horse populations of Eurasia.