Open AccessAn quantificational method for 51 pesticide residues determination in Pu'er tea by LC-MS/MS
Author(s) -
Xudong Wu,
Zeyu Chen,
Jiankang Zhang,
Man HongPing,
Che Tao,
Sheng Lv,
Jingchun Mao
Publication year - 2017
Publication title -
electronics science technology application
Language(s) - English
Resource type - Journals
eISSN - 2251-2608
pISSN - 2424-8460
DOI - 10.18686/esta.v4i1.33
Subject(s) - chromatography , chemistry , acetamiprid , residue (chemistry) , pesticide residue , pesticide , european union , detection limit , imidacloprid , liquid chromatography–mass spectrometry , selected reaction monitoring , tandem mass spectrometry , mass spectrometry , agronomy , biology , economic policy , biochemistry , business
Pesticide residues in tea is a major issue due to their widely used in tea cultivation. Thus, to protect consumers, an appropriate method for determination of their residues in tea should be done. In this study, a method for the simultaneous determination of 51 pesticides in tea was developed and validated. The tea sample was extracted by acetonitrile and purified SPE clearnet TPT column followed by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) with multi-reaction monitoring (MRM) mode. The method was validated according to the linearity, limit of detention, precision, the percentage of recovery at three different spike levels. The linear concentration range used was 10-100ng/ mL, the square of Correlation coefficient r2 was more than >0.995. Recoveries were adequate being in the acceptable range of 72-89% and RSD of <19 % for all the analytes at three level of 0.01,0.05 and 0.11mg/kg, the LOD of all chemicals from 0.001 mg/kg to 0.01 mg/kg .The method was applied for the determination for 400 tea samples collected from Pu'er which contain green tea and black tea. Among the analyzed samples , 36 % samples had Imidacloprid and 25% sample contain Acetamiprid, which were at a level below the European Union maximum residue levels (EU-MRLs). The information would be beneficial for Pu'er tea exporters.