Effect of Incubation with Dimethyl Sulfoxide on the Mitotic Cycle of Cell Culture of Rabbit Dermal Papilla

At cryopreservation, the issue of preserving the integrity and cytogenetic stability of dermal papilla (DP) cells of the hair follicle is topical. The study examined the effect of various concentrations of the cryoprotectant dimethyl sulfoxide (DMSO) (5-15%), as well as its combination with 5% bovine serum albumin (BSA), on the mitotic index (MI) and the number of pathological mitoses in the cell culture of DP on the first and second passages. It has been established that the treatment of cells with DMSO in concentrations of 10% and above leads to a significant increase in the number of pathological mitosis. This is accompanied by the decrease in MI (when processing concentrations of DMSO below 10%) at the first passage or a complete cessation of proliferation at the second passage (when processing concentrations of DMSO above 10%). The combination of 5% DMSO with BSA is optimal for minimizing toxic effects on the genetic apparatus of DP cells and can be used as part of cryopreservation media.