Open AccessmiR-493 mediated DKK1 down-regulation confers proliferation, invasion and chemo-resistance in gastric cancer cells
Author(s) -
Xiaoting Jia,
Nan Li,
Cong Peng,
Yingen Deng,
Jia Wang,
Min Deng,
Mengji Lu,
Jun Yin,
Guopei Zheng,
Haiying Liu,
Zhiwei He
Publication year - 2016
Publication title -
oncotarget
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.373
H-Index - 127
ISSN - 1949-2553
DOI - 10.18632/oncotarget.6951
Subject(s) - dkk1 , in vivo , cancer research , metastasis , in vitro , cancer , cancer cell , biology , chemistry , medicine , microbiology and biotechnology , signal transduction , wnt signaling pathway , biochemistry
In the present study, we demonstrated that the levels of DKK1 were decreased in serums and tissues of GC. DKK1 levels inversely correlated with tumor class, TNM stage, distant metastasis and lymph node metastasis of GC. GC patients with low DKK1 levels had a poor overall survival. DKK1 inhibited the proliferation of GC cells in vitro and in vivo. DKK1 also inhibited invasion, but enhanced chemo-sensitivity of GC cells. Mechanically, miR-493 levels increased in GC and directly targeted and down-regulated DKK1 expression. In agreement, miR-493 promoted proliferation of GC cells in vitro and in vivo. MiR-493 also promoted invasion and chemo-resistance of GC cells. However, DKK1 overexpression reversed the effects of miR-493 on proliferation, invasion and chemo-sensitivity. Thus, our results provide new insight for the role of miR-493/DKK1 axis in GC.