Open AccessGenome-wide analysis of DNA methylation and gene expression patterns in purified, uncultured human liver cells and activated hepatic stellate cells
Author(s) -
Adil El Taghdouini,
Anita L. Sørensen,
Andrew H. Reiner,
Mar Coll,
Stefaan Verhulst,
Inge Mannaerts,
Cristina Ionica Øie,
Bård Smedsrød,
Mustapha Najimi,
Étienne Sokal,
Aernout Luttun,
Pau Sancho–Bru,
Philippe Collas,
Leo A. van Grunsven
Publication year - 2015
Publication title -
oncotarget
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.373
H-Index - 127
ISSN - 1949-2553
DOI - 10.18632/oncotarget.4925
Subject(s) - dna methylation , epigenetics , biology , hepatic stellate cell , chromatin immunoprecipitation , transcriptome , methylated dna immunoprecipitation , chromatin , microbiology and biotechnology , epigenomics , regulation of gene expression , histone , gene expression , methylation , promoter , cancer research , gene , genetics , endocrinology
Liver fibrogenesis - scarring of the liver that can lead to cirrhosis and liver cancer - is characterized by hepatocyte impairment, capillarization of liver sinusoidal endothelial cells (LSECs) and hepatic stellate cell (HSC) activation. To date, the molecular determinants of a healthy human liver cell phenotype remain largely uncharacterized. Here, we assess the transcriptome and the genome-wide promoter methylome specific for purified, non-cultured human hepatocytes, LSECs and HSCs, and investigate the nature of epigenetic changes accompanying transcriptional changes associated with activation of HSCs.