Open AccessGenome-wide analysis of the human malaria parasitePlasmodium falciparumtranscription factor PfNF-YB shows interaction with a CCAAT motif
Author(s) -
Wânia Rezende Lima,
David Martins,
Kleber Simônio Parreira,
Pedro Scarpelli,
Miriam S. Moraes,
Pantelis Topalis,
Ronaldo Fumio Hashimoto,
Célia Regina da Silva Garcia
Publication year - 2017
Publication title -
oncotarget
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.373
H-Index - 127
ISSN - 1949-2553
DOI - 10.18632/oncotarget.23053
Subject(s) - plasmodium falciparum , malaria , transcription factor , humanities , biology , gene , genome , microbiology and biotechnology , genetics , art , immunology
Little is known about transcription factor regulation during the Plasmodium falciparum intraerythrocytic cycle. In order to elucidate the role of the P. falciparum (Pf)NF-YB transcription factor we searched for target genes in the entire genome. PfNF-YB mRNA is highly expressed in late trophozoite and schizont stages relative to the ring stage. In order to determine the candidate genes bound by PfNF-YB a ChIP-on-chip assay was carried out and 297 genes were identified. Ninety nine percent of PfNF-YB binding was to putative promoter regions of protein coding genes of which only 16% comprise proteins of known function. Interestingly, our data reveal that PfNF-YB binding is not exclusively to a canonical CCAAT box motif. PfNF-YB binds to genes coding for proteins implicated in a range of different biological functions, such as replication protein A large subunit (DNA replication), hypoxanthine phosphoribosyltransferase (nucleic acid metabolism) and multidrug resistance protein 2 (intracellular transport).