Open AccessFAM83D, a microtubule-associated protein, promotes tumor growth and progression of human gastric cancer
Author(s) -
Minlu Huang,
Xianghua Ma,
Hui Shi,
Lei Hu,
Zhiyuan Fan,
Li Pang,
Fan Zhu,
Xiao Yang,
Wei Xu,
Binya Liu,
Zhenggang Zhu,
Chen Li
Publication year - 2017
Publication title -
oncotarget
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.373
H-Index - 127
ISSN - 1949-2553
DOI - 10.18632/oncotarget.20157
Subject(s) - cancer , gene knockdown , cancer research , metastasis , cell cycle , cell growth , medicine , tumor progression , mitosis , cancer cell , biology , pathology , cell culture , microbiology and biotechnology , genetics
FAM83D , a microtubule-associated protein (MAP), is overexpressed in diverse types of human cancer. The expression and critical role of FAM83D in human gastric cancer (GC), however, remains largely unknown. Here, we conducted molecular, cellular and clinical analyses to evaluate the functional link of FAM83D to GC. FAM83D expression was elevated in gastric tumors, and its expression strongly correlated with lymph node metastasis and TNM stage. In addition, over-expression of FAM83D in GC cell lines enhanced cell proliferation, cycle progression, migration, invasion, as well as tumor growth and metastatic dissemination in vivo . Furthermore, FAM83D exhibited a strong cell cycle correlated expression. The knockdown of FAM83D inhibited the regrowth of microtubules in GC cells. FAM83D was co-immunoprecipitated with HMMR, TPX2, and AURKA, a set of drivers of mitosis progression. Taken together, our results demonstrate FAM83D as an important player in the development of human gastric cancer, and as a potential therapeutic target for the treatment of cancer.