Open AccessFunctional polymorphisms in pre-miR146a and pre-miR499 are associated with systemic lupus erythematosus but not with rheumatoid arthritis or Graves’ disease in Mexican patients
Author(s) -
Isidro AlemánÁvila,
Mayra Jiménez-Morales,
Olga Beltrán-Ramírez,
Rosa Elda Barbosa-Cobos,
Silvia Jiménez-Morales,
Fausto SánchezMuñoz,
Guillermo Valencia-Pacheco,
Luís M. Amezcua-Guerra,
Yaneli Juárez-Vicuña,
Dulce Milagro Razo-Blanco Hernández,
María Concepción Aguilera-Cartas,
Ricardo F. López-Villanueva,
Oscar PeraltaZaragoza,
Carlos Alfonso Tovilla-Zárate,
Julián RamírezBello
Publication year - 2017
Publication title -
oncotarget
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.373
H-Index - 127
ISSN - 1949-2553
DOI - 10.18632/oncotarget.19621
Subject(s) - rheumatoid arthritis , medicine , disease , immunology , autoimmune disease , systemic disease , autoimmunity , graves' disease , connective tissue disease , arthritis , dermatology
Recently, different microRNA (miRNA) gene polymorphisms have been evaluated in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and Graves' disease (GD). In the present study, we examined three single-nucleotide polymorphisms (SNPs) located in the pre-miR-146a (rs2910164G/C), pre-miR-196a-2 (rs11614913C/T), and pre-miR-499 (rs3746444A/G) genes. Our study population included 900 Mexican patients with RA, SLE, or GD, as well as 486 healthy control individuals with no family history of inflammatory or autoimmune diseases. Genotyping was performed using TaqMan probes and a 5' exonuclease assay. None of the investigated SNPs were associated with RA or GD susceptibility under any genetic model (co-dominant, recessive, or dominant). Genotype and allele frequencies of the miR-196a-2 rs11614913C/T polymorphism were similar between SLE cases and controls. In contrast, the miR-146a rs2910164G/C and miR-499 rs3746444A/G polymorphisms were associated with SLE susceptibility. These SNPs were not associated with lupus nephritis (LN). Our results suggest that polymorphisms in miR-146a, miR-196a-2 , and miR-499 are not associated with RA or GD susceptibility. This is the first report documenting that the miR-146a rs2910164G/C and miR-499 rs3746444 polymorphisms are associated with SLE susceptibility but not with LN.