Open AccessGADD45β, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens
Author(s) -
Xinheng Zhang,
Yan Zhang,
Xinjian Li,
Wencheng Lin,
Zhenkai Dai,
Yongde Yan,
Piaopiao Lu,
Weiguo Chen,
Huanmin Zhang,
Feng Chen,
Jingyun Ma,
Qingmei Xie
Publication year - 2016
Publication title -
oncotarget
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.373
H-Index - 127
ISSN - 1949-2553
DOI - 10.18632/oncotarget.12027
Subject(s) - biology , gene , virology , virus , avian leukosis , immunosuppression , rna , viral replication , tumor virus , immunology , genetics
Avian leukosis virus subgroup J (ALV-J) is a retroviruses that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that promote resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens (F3 generation). RNA-sequencing (RNA-Seq) of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens (compared to the susceptible ones). We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45β inhibited ALV-J replication. GADD45β could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections.