Open AccessProteomic identification of cyclophilin A as a potential biomarker and therapeutic target in oral submucous fibrosis
Author(s) -
Yao Yuan,
Xiaohui Hou,
Hui Feng,
Rui Li,
Hao Xu,
Wang Gong,
Jing Deng,
Chang Sun,
Yijun Gao,
Jun Peng,
Yongming Wu,
Li Jiang,
Changyun Fang,
Qianming Chen
Publication year - 2016
Publication title -
oncotarget
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.373
H-Index - 127
ISSN - 1949-2553
DOI - 10.18632/oncotarget.11254
Subject(s) - oral submucous fibrosis , medicine , oral lichen planus , pathology , oral and maxillofacial pathology , dentistry
Oral submucous fibrosis (OSF) is a pre-cancerous lesion, which is characterized by fibrosis of the oral submucosa. Despite large body of studies focusing on this disease, the molecular mechanisms underlying the progression of OSF remained unclear. In this study, 2-DE-based proteomic approaches were employed to identify the differently expressed proteins between OSF and normal tissues. In total, 88 proteins were identified with altered expression levels, including CypA. Upregulation of CypA was further validated through immunohistochemistry staining combined with Q-PCR and western blot by using clinical samples. Statistical analyses reveal that CypA expression level is correlated to the progression of OSF. Finally, functional study reveals a pro-proliferative property of CypA in fibroblast cells by using multiple in vitro models. The present data suggest that CypA might be a potential biomarker and therapeutic target for OSF, and will lead to a better understanding of OSF pathogenesis.