Open AccessFNDC3B promotes cell migration and tumor metastasis in hepatocellular carcinoma
Author(s) -
Chin-Hui Lin,
Ying-Feng Lin,
YingChun Chen,
Chi Chang Liao,
Yuh–Shan Jou,
Ming Hsu,
ChianFeng Chen
Publication year - 2016
Publication title -
oncotarget
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.373
H-Index - 127
ISSN - 1949-2553
DOI - 10.18632/oncotarget.10374
Subject(s) - hepatocellular carcinoma , metastasis , cancer research , gene knockdown , medicine , hccs , cell migration , cancer , oncogene , oncology , cell , biology , cell culture , cell cycle , genetics
Recurrence and metastasis are common in hepatocellular carcinoma (HCC) and correlate with poor prognosis. We investigated the role of fibronectin type III domain containing 3B (FNDC3B) in HCC metastasis. Overexpression of FNDC3B in HCC cell lines enhanced cell migration and invasion. On the other hand, knockdown of FNDC3B using short-hairpin RNA reduced tumor nodule formation in both intra- and extra-hepatic metastasis. High levels of FNDC3B were observed in metastatic HCCs and correlated with poor patient survival and shorter recurrence time. Mutagenesis and LC-MS/MS analyses showed that FNDC3B promotes cell migration by cooperating with annexin A2 (ANXA2). Furthermore, FNDC3B and ANXA2 expression correlated negatively with patient survival. Our results indicate that FNDC3B behaves like an oncogene by promoting cell migration. This suggests FNDC3B could serve as a biomarker and therapeutic target for HCC metastasis.