Open AccessmiR-345-5p regulates adipogenesis via targeting VEGF-B
Author(s) -
Xiaofeng Liu,
Yun He,
Z.L. Feng,
Jianjian Sheng,
Dong Ao,
Meiying Zhang,
Lingling Cao
Publication year - 2020
Publication title -
aging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.473
H-Index - 90
ISSN - 1945-4589
DOI - 10.18632/aging.103649
Subject(s) - adipogenesis , adipocyte , vascular endothelial growth factor , microrna , transcription factor , microbiology and biotechnology , downregulation and upregulation , chemistry , growth factor , biology , biochemistry , gene , vegf receptors , adipose tissue , cancer research , receptor , mesenchymal stem cell
Adipocyte differentiation involves a series of highly synergistic processes, including clone amplification, proliferation arrest, and terminal differentiation. However, the mechanisms that control these different steps remain unclear. Emerging studies support that miRNAs play an important role in regulating adipogenesis. In this study, we found that the expression of miR-345-5p decreased during adipogenic differentiation, and overexpression of miR-345-5p reduced lipid accumulation in adipocytes and the expression of adipocyte related genes essential to lipogenic transcription, fatty acid synthesis and fatty acid transport. In addition, miR-345-5p directly targeted the 3'UTR of vascular endothelial growth factor B, and miR-345-5p mimic inhibited the expression of vascular endothelial growth factor B at both mRNA and protein levels. In conclusion, our results demonstrate that miR-345-5p inhibits adipocyte differentiation via targeting vascular endothelial growth factor B.