Open AccessAbout the creation of sterile lines of sweet pepper in breeding for heterosis
Author(s) -
С.В. Королева,
Natalia Poliakova,
O.G. Pistun
Publication year - 2020
Publication title -
ovoŝi rossii
Language(s) - English
Resource type - Journals
eISSN - 2618-7132
pISSN - 2072-9146
DOI - 10.18619/2072-9146-2020-5-38-42
Subject(s) - greenhouse , sterility , horticulture , heterosis , growing season , biology , pepper , capsicum annuum , agronomy , environmental science , botany , hybrid
Relevance . Development of valuable sterile lines of sweet pepper using nuclear-cytoplasmic male sterility is associated with the search for stable sterility maintainers in collection material. Material and methods . The purpose of the research is to optimize the identification of the desired genotypes and accelerate the development of sterile lines using artificial climate chambers. The research material was a sterile line msTol55, which under the conditions of a film greenhouse showed partial fertility at the beginning or at the end of the growing season. The place of cultivation is artificial climate chamber, a film spring greenhouse.50-day-old seedlings were planted in pots on 10.01, the period of growing plants in the chamber - until 20.04, then the adult plants were transplanted into a spring film greenhouse. According to the traditional technology, the cassette seedlings of the same line were planted in the greenhouse on 15.04. The number of tested plants is 16-20 pcs. Growing mode in the chamber: 12 hours were illuminated with DRLV lamps, while the temperature was at the level of 26-28°C, from 20.00 to 8.00 the lamps were not illuminated, the temperature was 14-15°C. The actual sterility / fertility of the flowers was determined by the level of contamination of the fruits at the beginning of their formation. Results . The maintained temperature regime for growing in an artificial climate chamber made it possible to identify sterile (30%) and partially fertile plants (70%) in the first 2 weeks of flowering. When adult plants were transplanted into a spring greenhouse, the number of sterile plants increased to 56%. When growing the line msTol55 in a spring greenhouse using the usual technology, partial setting of seeded and little seeded fruits on 60% of the plants was observed from the third decade of May to the second decade of July, then all plants set only non-seeded fruits. Based on the results obtained, it is advisable to identify the breeding material for the Rf gene in the ACC, since the proposed growing regime serves as a provocative background and allows for more effective control over the manifestation of sterility.